|
KMID : 1007520230320010059
|
|
Food Science and Biotechnology
2023 Volume.32 No. 1 p.59 ~ p.69
|
|
|
Development of real-time PCR based molecular markers for two medicinal herb Artemisia species A. capillaris and A. iwayomogi
|
|
Kim Yea-Dam
Uh Yo-Ram
Lee Jee-Hyun
|
|
|
Abstract
|
|
|
|
Artemisia capillaris and Artemisia iwayomogi are well-known herbal medicines which are used as hepatotherapeutic drugs. These two herbal species can be confused with each other, owing to their morphological similarity and similar Korean common names of “Injinho” and “Haninjin,” respectively. Molecular markers to distinguish between the two plants were developed. Six primer sets were designed and verified, and their efficiencies were found to range from 90.28 to 98.29%. The developed primer sets had significant correlation coefficient values between the cycle threshold values and the logarithm of DNA concentration for their target species (R2?>?0.98), with slopes ranged from ? 3.3637 to ? 3.5793. The specificity of the quantitative polymerase chain reaction (qPCR) was confirmed with 14 other species. Additionally, 16 commercial medicinal herbs and 40 blind samples were tested to evaluate their reliability. Collectively, the findings indicate that developed qPCR-based target-specific primer sets have potential applicability toward protection of consumers’ rights.
|
|
|
KEYWORD
|
|
|
Artemisia capillaris, Artemisia iwayomogi, Medical herb, Species identification, SYBR-GREEN real-time PCR
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|